Cell Biology

Membrane Transport: Diffusion, Osmosis & Active Transport

Compare passive and active transport mechanisms across cell membranes. Covers simple diffusion, facilitated diffusion, osmosis, and the sodium-potassium pump.

V
Vectora Team
STEM Education
8 min read
2025-10-03

Why is Membrane Transport Important?

Cells must control what enters and exits. The phospholipid bilayer is selectively permeable — small nonpolar molecules pass freely, but ions and large molecules need help.

Learning Goals:

  1. Distinguish passive from active transport.
  2. Describe four transport mechanisms.
  3. Predict the direction of water movement using water potential.

Comparison Table

FeatureSimple DiffusionFacilitated DiffusionOsmosisActive Transport
EnergyNoneNoneNoneATP required
DirectionHigh → Low conc.High → Low conc.High → Low Ψ\PsiLow → High conc.
ProteinsNoneChannel/carrierAquaporinsCarrier pumps
MoleculesO₂, CO₂, steroidsGlucose, ionsWater onlyNa⁺, K⁺, Ca²⁺

Osmosis & Water Potential

Osmosis is the net movement of water from a region of higher water potential (Ψ\Psi) to lower water potential through a partially permeable membrane.

SolutionRelative [solute]Water movementCell effect
HypotonicLower outsideWater enters cellAnimal: lyses. Plant: turgid
IsotonicEqualNo net movementNormal
HypertonicHigher outsideWater leaves cellAnimal: crenates. Plant: plasmolysed

Worked Examples

Example 1: Red blood cell in distilled water

Distilled water is hypotonic. Water enters by osmosis → cell swells → lysis (bursts). Plant cells don't burst because of the rigid cell wall — they become turgid.

Example 2: Why do Na⁺/K⁺ pumps use ATP?

They transport ions against their concentration gradient (3 Na⁺ out, 2 K⁺ in). This requires energy from ATP hydrolysis.


Common Mistakes

  1. "Osmosis is diffusion of all molecules" — Osmosis specifically refers to water movement through a partially permeable membrane.
  2. Confusing hypotonic and hypertonic — Hypotonic = lower solute concentration relative to the cell.
  3. Forgetting facilitated diffusion is still passive — No ATP is used; it goes down the concentration gradient.

Exam Tips

  • Define transport type by three criteria: energy source, direction, protein involvement.
  • For osmosis questions, always state the water potential gradient direction.
  • Active transport example: Na⁺/K⁺ pump, gut absorption of glucose.